Intrinsic fluorescence can be measured on the J or J Circular Dichroism spectrophotometers using the scanning emission monochromator (FMO) and emission detector (FDT) to acquire fluorescence emission spectra. Alternatively, high-pass filters can be used to select specific excitation and emission www.doorway.ruted Reading Time: 3 mins. FP/ Fluorescence Detector The FP fluorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature-regulated cell body has led to the highest sensitivity with stable detection. The JASCO Model FP Intelligent Fluorescence Detector is recognized as the industry’s most sensitive detector with a signal-to-noise ratio of better than for the Raman water peak. The FP covers a wide wavelength range both for excitation and emission from to nm (~ nm with an optional PMT) with proven www.doorway.ru: Jasco.
To provide greater accuracy, Jasco developed a calibrated light source – D2 and a calibrated detector for the FP series, which will cover the full wavelength range with directly observed correction data. Calibrated light source – D2 SID Calibrated detector n m nm Rhodamine B nm nm Calibrated light source - WI. Figure 1 shows the JASCO SFC system equipped with UV/Visible (UV) and fluorescence (FL) detectors used in this experiment. Figure 2 also shows the schematic diagram of this system. This system enables method scouting analysis to be performed on several mobile phases and columns. Auto-Gain automatically adjusts the gain of a signal from the detector, so that the S/N is optimized throughout the entire scan range for spectral or time course measurements. Since quantum yield measurements can produce peaks that vary greatly in fluorescence intensity, the auto-gain function assures the accuracy of the measurement.
JASCO UV and Fluorescence detectors can be setup in software to specify wavelength, autozero controls, detector outputs as well as a time program to specify. Important Notice: Other accessories, manuals, cables, calibration data, software, etc. are not included with this equipment unless listed in the above stock. Contact your local JASCO distributor for instrument servicing. In addition, contact your JASCO Detector for fluorescence configuration.
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